RNA VIRAL INFECTION RT-PCR Kit

Catalog #3401

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GenoSensor RNA Viral Infection RT-PCR Kit User’s Manual Cat # 3401

The RNA Virial Infection RT-PCR kit is designed to introduce students to the RNA viral world and RT-PCR – an RNA amplification technology.

• Perform an RNA amplification via RT-PCR technique

• Verify presence of RT-PCR amplified RNA by gel electrophoresis

• Fast and simple protocol for easy integration into biotechnology lessons

In this lab, students play the role of the diagnostician in the following scenario. Patients arrive at the emergency room with fever and cough. An RNA viral infection is suspected and must be confirmed or ruled-out from patient samples. A short RNA is used to emulate the virus; no actual viral particles are used.

Kit Components Included:Qty
RNA VIRAL INFECTION RT-PCR KIT (Catalog# 3401)
--Sample A,60 µL for each DNA included
--Sample B,60 µL for each DNA included
--Sample C60 µL for each DNA included
--Sample Unknown DNA60 µL for each DNAincluded
--2X PCR Master Mix (includes primers and enzyme)270 µLincluded
--ddH2O30 µLincluded
--DNA Ladder30 µLincluded
Additional Lab Materials and Equipment Needed:
--Heat Block or Water Bath -
--Microcentrifuge -
--Vortex -
--Electrophoresis & Reagents (agarose, -
----------running buffer and DNA dye) -
--Electrophoresis Aparatus -
--UV Lamp / UV Light Box -