The students are medical professionals, in an emergency room, trying to save a patient's life by identifying a food-borne illness-causing microbe. The kit includes three different plasmids, each representing a possible microbe, and an "unknown" sample from the patient. After completing the experiment, students will not only find out the answer, but also understand restriction digests or PCR, gel electrophoresis, and genetic concepts associated with this experiment.
Each kit is good for 24 students with 6 reactions worth of material for each of the samples (The unknown and Sample A, B and C) and 24 reactions of a 2X Master Mix.
Scenario: A patient came into the emergency room and was diagnosed with food poisoning. The doctors would like to know which illness-causing microbe is responsible. A fluid sample is retrieved and tested for several common microbes.
Multiple Plasmid DNA: Plasmid DNA is used to emulate the genetic diversity seen in illness-causing microbes. Four plasmid samples are included in the kit, one representing DNA from the infectious microbe taken from the patient, and three for comparison against the sample taken from the patient.
Ease of Use: The protocol is easy to understand and follow. Kit components are ready-made and easy to set up, reducing preparation time for instructors.
Learning Applications: The kit teaches the fundamental concepts of genetic diversity and real world applications in medical diagnoses, while introducing students to restriction digests or PCR and gel electrophoresis.
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