The GenoExplorer™ miRNA quantitative RT-PCR kit has
been developed and optimized to amplify specific microRNAs,
allowing real time quantitation of miRNA expression levels.
This system can achieve
Cat # |
Product Name |
Description |
Size |
Price |
2001 |
GenoExplorer™ miRNA qRT-PCR Kit-100 |
Provides complete set for miRNA quantitation
including first-strand cDNA synthesis and SYBR® Green
I qPCR reagent mix. (primers not included, see Cat# 2003
and 2004) |
100
reactions |
US$290 |
2002-10 |
GenoExplorer™ miRNA First-Strand cDNA
Core Kit-10 |
Provides necessary core components to generate
miRNA first-strand cDNA for miRNA expression quantitation.
|
10
reactions |
US$210 |
2002-50 |
GenoExplorer™ miRNA First-Strand cDNA Core Kit-50 |
Provides necessary core components to generate miRNA
first-strand cDNA for miRNA expression quantitation. |
50
reactions |
US$650 |
2003 |
GenoExplorer™ miRNA qPCR Primer Sets |
Provides specific primer pair for miRNA quantitation
analysis (3µM each). Suggest to combine with GenoExplorer™
miRNA qRT-PCR Kit-100 (Cat# 2001) and reference primer
sets (Cat# 2004). Refer miRBase
for available genes and primers.
When ordering, enter species-mir-number. e.g.
hsa-miR-125a, mmu-mir-124-1-precursor
|
|
100 µl |
US$60 |
2004 |
GenoExplorer™ miRNA qPCR Reference Primer Sets
|
Reference primer as an internal control reference
(3µM each). Suggest to combine with GenoExplorer™
miRNA qRT-PCR Kit-100 (Cat# 2001) and GenoExplorer™
miRNA qPCR Primer Sets (Cat# 2003)
Selection of human-U6, human-U44, human-5SrRNA;
mouse-U6, mouse-5SrRNA; rat-U6, rat-5SrRNA; Drosophila-U6
and 5SrRNA |
|
100 µl |
US$60 |
2005 |
GenoExplorer™ 2X qPCR Mix |
2X SYBR® Green I qPCR mix, specifically for miRNA
and small RNA amplification and quantification, containing
all PCR reagents and polymerase |
750 µl |
US$160 |
• C Li et al. MicroRNA-29b-2-5p inhibits
cell proliferation by directly targeting Cbl-b in pancreatic
ductal adenocarcinoma. BMC Cancer. 2018; 18:681-94
• Ji-Su Mo et al. MicroRNA 429 Regulates Mucin Gene
Expression
and Secretion in Murine Model of Colitis. J Crohn's Colitis
2016; Jan; 837-49
• Kim JM et al. Inhibition of Let7c
microRNA is neuroprotective in a rat intracerebral hemorrhage
model. PLoS One. 2014; Jun 24;9(6):e97946.
• Colin C. Pritchard et al. MicroRNA
profiling: approaches and considerations. Nature 2012; 13:358-69
• Park JH et al. Murine hepatic miRNAs
expression and regulation of gene expression in diet-induced
obese mice. Mol. Cells 2011; 31:33-38.
• Foss KM et al. miR-1254 and miR-574-5p: serum-based
microRNA biomarkers for early-stage non-small cell lung
cancer. J Thoracic Oncol 2011; 6(3):482-8.
• Arora S et al. MicroRNA-328 is associated with
(non-small) cell lung cancer (NSCLC) brain metastasis and
mediates NSCLC migration. Intl J Cancer 2011; 128(11): Mar
29.
• Leeper NJ et al. MicroRNA-26a is a novel regulator
of vascular smooth muscle cell function. J Cellular Physiol
2011; 226(4):1035-43.
• Lu C and Souret F. High-throughput approaches for
miRNA expression analysis. Plant MicroRNAs 2010; 592:107-25.
• Mouillet JF et al. MiR-205 silences MED1 in hypoxic
primary human trophoblasts. FASEB J 2010; 24(6):2030-9.
• Ranade AR et al. MicroRNA 92a-2*: a biomarker predictive
for chemoresistance and prognostic for survival in patients
with small cell lung cancer. J Thoracic Oncol 2010; 5(8):1273-8.
• Li G et al. Modulation of inflammatory markers
by miR-146a during replicative senescence in trabecular
meshwork cells. Invest Ophthalmol Vis Sci 2010; Jan 6
• Hanoun N et al. The silencing of microRNA 148a
production by DNA hypermethylation is an early event in
pancreatic cancinogenesis. Clin Chem 2010; Apr 29
• Liang Z et al. Involvement of miR-326 in chemotherapy
resistance of breast cancer. Biochem Pharmacol 2010; 79(6):817-24
• Igoucheva O and Alexeev V MicroRNA-dependent regulation
of cKit in cutaneous melanoma. Biochem Biophys Res Comm
2009; 379(3):790-4.
• Liu X et al. MicroRNA-222 regulates cell invasion
by targeting matrix metalloproteinase 1 (MMP1) and manganese
superoxide dismutase 2 (SOD2) in tongue squamous cell carcinoma
cell lines. Cancer Genomics Proteomics 2009; 6(3):131-9.
• Nasser S et al. Identifying miRNA and imaging features
associated with metastasis of lung cancer to the brain.
IEEE 2009
• Blow N. Small RNAs: biology’s brave new world.
Nature Methods 2009; 6(3):232-5.
• Liu X et al. microRNA-138 suppresses invasion and
promotes apoptosis in head and neck squamous cell carcin.
Cancer Lett2009; 286(2):217-22
• Lee S-O et al. MicroRNA15a modulates expression
of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis
in a rat model of polycystic kidney diseases. J Clin Invest
2008; 18(11):3714-24
• Chang SS et al. miRNA alterations inhead and neck
squamous cell carcinoma. Int J Cancer 2008; 123,2791-7
• Park CS and Tang SJ. Regulation of microRNA Expression
by Induction of Bidirectional Synaptic Plasticity. J Mol
Neurosci. 2008; Nov 8
• Goff LA et al. The analysis of microRNAs in stem
cells. Stem Cell Res Therap 2008; 1:141-67.
• Chang HY et al. Microarray analysis of stem cells
and their differentiation. Essential stem cell methods 2008;
Chapter 12.
• Chen X-M et al. A cellular miRNA, let-7i, regulates
toll-like receptor 4 expression and contributes to cholangiocyte
immune responses against Cryptosporidium parvum infection.
J. Biol. Chem. 2007; July 27
• Baroukh N etc. MicroRNA-124a regulates Foxa2 expression
and intracellular signaling in pancreatic beta -cells lines.
J Biol Chem 2007; Apr 26
• Donker RB et al. The expression of argonaute2 and
related microRNA biogenesis proteins in normal and hypoxic
trophoblasts. Mol Hum Reprod 2007; 13(4):273-9.
• Mott JL et al. Mir-29 regulates Mcl-1 protein expression
and apoptosismer-29 downregulates Mcl-1. Oncogene 2007;
26:6133-40.
• Weston MD et al. MicroRNA gene expression in the
mouse inner ear.
Brain Res 2006; 1111(1)95-104