What's in my water? - The water we drink is put through large amounts of testing to make sure that
it's safe for us to drink. What are those tests, though, and what do they look for? The Water Analysis
kit gives students the opportunity to test water samples from their own environment, and through PCR detect
the presence of bacterial contaminants.
- Real world applications - The bacteria tested for in this kit are the same that are tested for in initial water contamination tests.
- Fun for students - Any water sample can be used, anything from the puddle outside to the toilet.
- Continued learning - Students don't just have to stop at the PCR test. Other tests can be performed, and students can develop their own ideas of how the water might be made cleaner.
|
|
| Kit Components Included:
| Qty
|
|
| PCR Reaction Components |
| --2X PCR Master Mix (includes primers) |
300 µL |
included |
| --Positive Control water |
30 µL |
included |
| --Negative Control water |
30 µL |
included |
| Gel Electrophoresis Components |
| --DNA Ladder |
30 µL |
included |
| Additional Lab Materials Needed: |
| --Microcentrifuge |
|
- |
| --PCR Thermal Cycler |
|
- |
| --Micropipettes & Tips |
|
- |
| --PCR Tubes |
1/sample |
- |
| --Microcentrifuge Tubes |
1/sample |
- |
| --Vortex |
|
- |
| --Heat Block or Water Bath |
|
- |
| --Agarose for ~0.8% - 1% gel |
|
- |
| --TAE Buffer |
|
- |
| --DNA Dye (recommended in gel, UV visualized dye) |
|
- |
| --Electrophoresis Aparatus |
|
- |
| --UV Lamp / UV Light Box |
|
- |
| --Scissors and Tweezers |
|
- |
| Cat #
| Product Name
| Description
| Size
| List Price
|
5001
|
Water Analysis - PCR Kit |
Full kit, as detailed in kit components |
30 rxns
|
$140 |
References
YouTube
video shows Water Analysis kit process.
Bej AK, McCarty SC, Atlas RM. Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: comparison with defined substrate and plating methods for water quality monitoring. Appl Environ Microbiol. 1991;57(8):2429-2432.
Annie Rompre' et al. Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. Journal of Microbiological Methods 49 (2002)
