The EduPrimer™
DNA Profiling Kit is specifically designed to introduce students to the principles
and applications of PCR, focusing on its real-life use of DNA profiling
in forensics and disease research. The core kit is designed with 30
reactions total, meant for 24 student reactions and 6 control reactions.
- Highly variable results between students – The microsattelite region targeted with this kit makes it an excellent example of genetic elements that can be used for identification.
- Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.
- Quick protocol involving isolation of students' DNA, PCR and electrophoresis, all completed within a 2.5 - 3 hour time frame.
|
|
| Kit Components Included:
| Qty
|
|
| DNA Isolation Components |
| --Solution A (Lysis buffer) |
6 mL |
included |
| --Solution B (Neutralizer) |
0.6 mL |
included |
| --Cotton Swabs |
28 swabs |
included |
| PCR Reaction Components |
| --2X PCR Master Mix (includes primers) |
300 µL |
included |
| --Positive Control DNA(Heterozygous max and minimum size) |
30 µL |
included |
| --Negative Control |
30 µL |
included |
| Gel Electrophoresis Components |
| --DNA Ladder |
30 µL |
included |
| Additional Lab Materials Needed: |
| --Microcentrifuge |
|
- |
| --PCR Thermal Cycler |
|
- |
| --Micropipettes & Tips |
|
- |
| --PCR Tubes |
1/sample |
- |
| --Microcentrifuge Tubes |
1/sample |
- |
| --Vortex |
|
- |
| --Heat Block or Water Bath |
|
- |
| --Agarose for ~0.8% - 1% gel |
|
- |
| --TAE Buffer |
|
- |
| --DNA Dye (recommended in gel, UV visualized dye) |
|
- |
| --Electrophoresis Aparatus |
|
- |
| --UV Lamp / UV Light Box |
|
- |
| --Scissors and Tweezers |
|
- |
| Cat #
| Product Name
| Description
| Size
| List Price
|
3000
|
EduPrimer™ DNA Isolation Kit |
DNA Isolation components only (Solution A, B and Swabs)
|
30 rxns
|
$55 |
3003
|
EduPrimer™ VNTR DNA Profiling Kit |
Full kit, as detailed in kit components, using VNTR primers |
30 rxns
|
$165.25 |
3003R
|
EduPrimer™ VNTR PCR Reaction Kit |
PCR reaction components only.
|
30 rxns
|
$125 |
3003C
|
Positive Control for EduPrimer™ VNTR DNA
Profiling Kit |
Extra positive control DNA for the VNTR kit.
|
10 rxns
|
$30 |
References
YouTube
video shows VNTR DNA Profiling kit process.
Arizona State University also made a video describing their experience with the kit:
References
Wu, Kun-sheng et al. Detection of microsatellite polymorphisms without cloning. Nucleic Acids Research (1994)
John M. Butler, Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers, Second Edition, Academic Press, 2005.
