The EduPrimer™ DNA Profiling Kit is specifically designed to introduce students to the principles and applications of PCR, focusing on its real-life use of DNA profiling in forensics and disease research. The core kit is designed with 30 reactions total, meant for 24 student reactions and 6 control reactions
Highly variable results between students – The micro-satellite region targeted with this kit makes it an excellent example of genetic elements that can be used for identification.
Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.
Quick protocol involving isolation of students' DNA, PCR and electrophoresis, all completed within a 2.5 – 3-hour time frame.
|DNA Isolation Components|
|--Solution A (Lysis buffer)||6 mL||included|
|--Solution B (Neutralizer)||0.6 mL||included|
|--Cotton Swabs||28 swabs||included|
|PCR Reaction Components|
|--2X PCR Master Mix (includes primers)||300 µL||included|
|--Positive Control DNA(Heterozygous max and minimum size)||30 µL||included|
|--Negative Control||30 µL||included|
|Gel Electrophoresis Components|
|--DNA Ladder||30 µL||included|
Additional Lab Materials Needed:
|--PCR Thermal Cycler||-|
|--Micropipettes & Tips||-|
|--Heat Block or Water Bath||-|
|--Agarose for ~0.8% - 1% gel||-|
|--DNA Dye (recommended in gel, UV visualized dye)||-|
|--UV Lamp / UV Light Box||-|
|--Scissors and Tweezers||-|
Catalog & Options
|3000||EduPrimer™ DNA Isolation Kit||DNA Isolation components only (Solution A, B and Swabs)||30 rxns||$55|
|3003||EduPrimer™ VNTR DNA Profiling Kit||Full kit, as detailed in kit components, using VNTR primers||30 rxns||$165.25|
|3003R||EduPrimer™ VNTR PCR Reaction Kit||PCR reaction components only.||30 rxns||$125|
|3003C||Positive Control for EduPrimer™ VNTR DNA Profiling Kit||Extra positive control DNA for the VNTR kit.||10 rxns||$30|
Wu, Kun-sheng et al. Detection of microsatellite polymorphisms without cloning. Nucleic Acids Research (1994)
John M. Butler, Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers, Second Edition, Academic Press, 2005.