The students are asked to identify an unknown predatory animal that is killing calves on a nearby ranch. The kit includes three different DNA, each representing possible animals, and an "unknown" DNA sample left behind by the predator. After completing the experiment, students will not only find out the answer, but also understand restriction digests or PCR, gel electrophoresis, and genetic concepts associated with this experiment.
Each kit is good for 24 students with 6 reactions worth of material for each of the samples (The unknown and Sample A, B and C) and 24 reactions of a 2X Master Mix.
- Scenario: The rancher found hair left behind by the suspect predator. The students are asked to identify the responsible animal.
- Multiple Plasmid DNA: Plasmid DNA is used to emulate the genetic diversity seen in animals. Four plasmid samples are included in the kit, one representing DNA from the predator, and three for comparison against the predator sample.
- Ease of Use: The protocol is easy to understand and follow. Kit components are ready-made and easy to set up, reducing preparation time for instructors.
- Learning Application: The kit teaches the fundamental concepts of genetic diversity and real world applications in animal identification, while introducing students to restriction digests or PCR and gel electrophoresis.
|
| Kit Components Included:
| Qty
|
|
| Animal Identification: Catch a Predator Kit I (Catalog# 7005) |
| --Sample A, B, C, and Unknown DNA |
60 µL for each DNA |
included |
| --2X Res. Digest Master Mix (includes restriction enzymes and buffer) |
240 µL |
included |
| --DNA Ladder |
30 µL |
included |
| Animal Identification: Catch a Predator Kit II (Catalog# 7006) |
| --Sample A, B, C, and Unknown DNA |
60 µL for each DNA |
included |
| --2X PCR Master Mix (includes primers and enzyme) |
270 µL |
included |
| --ddH2O |
30 µL |
included |
| --DNA Ladder |
30 µL |
included |
| Additional Lab Materials and Equipment Needed: |
| --Heat Block or Water Bath |
|
- |
| --Microcentrifuge |
|
- |
| --Vortex |
|
- |
| --Electrophoresis & Reagents (agarose, |
|
- |
| ----------running buffer and DNA dye) |
|
- |
| --Electrophoresis Aparatus |
|
- |
| --UV Lamp / UV Light Box |
|
- |
| Cat #
| Product Name
| Description
| Size
| List Price
|
7005
|
Animal Identification: Catch a Predator Kit - I |
Restriction digest full kit, examining animal species, level 1 |
24 rxns
|
$138 |
7006
|
Animal Identification: Catch a Predator Kit- II |
PCR full kit, examining animal species, level 2 |
27 rxns
|
$140 |
3010
|
DNA ladder |
Extra DNA ladder |
100 µL
|
$42 |
References
YouTube
video shows Catch a predator kit process
Contact us if you want to order or have
more questions.
