Assay Performance and Technical Bulletin
The GenoExplorerTM microRNA kit offers several competitive
features:
- Most comprehensive probe content for both mature and
precursor miRNA
- Highly-sensitive platform
- Highly-specific platform
- Highly-reproducible platform
1. The GenoExplorerTM microRNA chip contains the most comprehensive
probe content for microRNA profiling. GenoExplorer microRNATM
chips offer the user the opportunity to perform differential
expression analyses between different samples, not only for
the mature miRNA, but also for the precursor miRNA.
The graph shows log2 ratios of mean signal data comparing two different human RNA samples (experimental and control) for 3 sets of probes for both mature and precursor miRNAs.
2. The GenoExplorer™ microRNA platform offers a highly
sensitive system for miRNA analysis.
Eight synthesized bacterial RNA transcripts
(30mer) were used for array hybridization. Spike experiment
was performed with all eight synthetic RNA according to a
predetermined spiking concentration matrix with a fixed amount
of 2 ug total RNA samples to generate a real background on
the array. The concentrations of the eight different spiking
RNAs in each sample cover 3.5 logs. The samples containing
different spiking RNA concentration are hybridized to different
array chips. The spiking signal intensities were normalized
against the signal intensity of the U6 positive control of
each chip. The result showed a low detection limit is 0.5
pM (equivalent to 10 attomoles) and a dynamic range of no
less than 3 logs.
3. The GenoExplorer™ microRNA platform also offers
a highly specific platform that can discriminate between sequences
with up to 94% homology.
The graph shows mean signal data for pairs of miRNA with
single-base and double-base mismatches using human brain RNA.
The first two pairs are single-base mismatches with 94% homology
and the last pair is a double-base mismatch with a homology
of 90%. All mismatches yield fold-changes greater than 10x.
4. The GenoExplorer™ microRNA platform yields highly
reproducible results with an average CV less than 15% between
experiments.
Back to GenoExplorer™ microRNA
Expression Analysis |
