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EduPrimer™ VNTR DNA Profiling Kit

Catalog #3003

Price: $150

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The EduPrimer™ DNA Profiling Kit is specifically designed to introduce students to the principles and applications of PCR, focusing on its real-life use of DNA profiling in forensics and disease research. The core kit is designed with 30 reactions total, meant for 24 student reactions and 6 control reactions.

  • Highly variable results between students The microsattelite region targeted with this kit makes it an excellent example of genetic elements that can be used for identification.

  • Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.

  • Quick protocol involving isolation of students' DNA, PCR and electrophoresis, all completed within a 2.5 - 3 hour time frame.
Kit Components Included: Qty
DNA Isolation Components
--Solution A (Lysis buffer) 6 mL included
--Solution B (Neutralizer) 0.6 mL included
--Cotton Swabs 28 swabs included
PCR Reaction Components
--2X PCR Master Mix (includes primers) 300 µL included
--Positive Control DNA(Heterozygous max and minimum size) 30 µL included
--Negative Control 30 µL included
Gel Electrophoresis Components
--DNA Ladder 30 µL included
Additional Lab Materials Needed:
--Microcentrifuge -
--PCR Thermal Cycler -
--Micropipettes & Tips -
--PCR Tubes 1/sample -
--Microcentrifuge Tubes 1/sample -
--Vortex -
--Heat Block or Water Bath -
--Agarose for ~0.8% - 1% gel -
--TAE Buffer -
--DNA Dye (recommended in gel, UV visualized dye) -
--Electrophoresis Aparatus -
--UV Lamp / UV Light Box -
--Scissors and Tweezers -
Cat # Product Name Description Size List Price
3000
EduPrimer™ DNA Isolation Kit

DNA Isolation components only (Solution A, B and Swabs)

30 rxns
$55
3003
EduPrimer™ VNTR DNA Profiling Kit

Full kit, as detailed in kit components, using VNTR primers

30 rxns
$150
3003R
EduPrimer™ VNTR PCR Reaction Kit

PCR reaction components only.

30 rxns

$105

3003C
Positive Control for EduPrimer™ VNTR DNA Profiling Kit

Extra positive control DNA for the VNTR kit.

10 rxns
$30
User Manual click to download Download
Single Page Protocol click to download Download (currently unavailable)
Videos

We've put together short, 4 1/2 minute video showing kit components and the protocol:

Arizona State University also made a video describing their experience with the kit:

References

Wu, Kun-sheng et al. Detection of microsatellite polymorphisms without cloning. Nucleic Acids Research (1994)

John M. Butler, Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers, Second Edition, Academic Press, 2005.

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