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Food Poisoning Kit I & II

Catalog #3201, 3202

Price: $138, $140

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The students are medical professionals, in an emergency room, trying to save a patient's life by identifying a food-borne illness-causing microbe. The kit includes three different plasmids, each representing a possible microbe, and an "unknown" sample from the patient. After completing the experiment, students will not only find out the answer, but also understand restriction digests or PCR, gel electrophoresis, and genetic concepts associated with this experiment.

Each kit is good for 24 students with 6 reactions worth of material for each of the samples (The unknown and Sample A, B and C) and 24 reactions of a 2X Master Mix.

  • Scenario: A patient came into the emergency room and was diagnosed with food poisoning. The doctors would like to know which illness-causing microbe is responsible. A fluid sample is retrieved and tested for several common microbes.
  • Multiple Plasmid DNA: Plasmid DNA is used to emulate the genetic diversity seen in illness-causing microbes. Four plasmid samples are included in the kit, one representing DNA from the infectious microbe taken from the patient, and three for comparison against the sample taken from the patient.
  • Ease of Use: The protocol is easy to understand and follow. Kit components are ready-made and easy to set up, reducing preparation time for instructors.
  • Learning Applications: The kit teaches the fundamental concepts of genetic diversity and real world applications in medical diagnoses, while introducing students to restriction digests or PCR and gel electrophoresis.
Kit Components Included: Qty
Food Poisoning Kit I (Catalog# 3201)
--Sample A, B, C, and Unknown DNA 60 µL for each DNA included
--2X Res. Digest Master Mix (includes restriction enzymes and buffer) 240 µL included
--DNA Ladder 30 µL included
Food Poisoning Kit II (Catalog# 3202)
--Sample A, B, C, and Unknown DNA 60 µL for each DNA included
--2X PCR Master Mix (includes primers and enzyme) 270 µL included
--ddH2O 30 µL included
--DNA Ladder 30 µL included
Additional Lab Materials and Equipment Needed:
--Heat Block or Water Bath -
--Microcentrifuge -
--Vortex -
--Electrophoresis & Reagents (agarose, -
----------running buffer and DNA dye) -
--Electrophoresis Aparatus -
--UV Lamp / UV Light Box -
Cat # Product Name Description Size List Price
3201
Food Poisoning Kit - I

Restriction digest full kit, examining food bacteria, level 1

24 rxns
$138
3202
Food Poisoning Kit - II

PCR full kit, examining food bacteria, level 2

27 rxns
$140

3010

DNA ladder Extra DNA ladder 100 µL

$42

User Manual I Cat# 3201 click to download Download
User Manual II Cat# 3202 click to download Download

References

YouTube video shows EduPrimer kit process and its simplicity.

Contact us if you want to order or have more questions.

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