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EduPrimer™ Alu DNA Profiling Kit

Catalog #3000 - 3010

Price: $150

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The EduPrimer™ DNA Profiling Kit is specifically designed to introduce students to the principles and applications of PCR, focusing on its real-life use of DNA profiling in forensics and disease research. The core kit is designed with 30 reactions total, meant for 24 student reactions and 6 control reactions.

  • Option between two dimorphic genes in the Alu family (TPA-25 and PV92) for a maximum of four shifted DNA bands per student, demonstrating the power of PCR, and teaching the techniques to utilize it.

  • Extremely simple and fast protocol for easy integration into classrooms such as Introductory or General Biology. Anyone can do it.

  • Quick protocol involving isolation of students' DNA, PCR and electrophoresis, all completed within a 2.5 - 3 hour time frame.
Kit Components Included: Qty
DNA Isolation Components
--Solution A (Lysis buffer) 6 mL included
--Solution B (Neutralizer) 0.6 mL included
--Cotton Swabs 28 swabs included
PCR Reaction Components
--2X PCR Master Mix (includes TPA25 or PV92 primers) 300 µL included
--Positive Control DNA(Heterozygous) 30 µL included
--Negative Control 30 µL included
Gel Electrophoresis Components
--DNA Ladder 30 µL included
Additional Lab Materials Needed:
--Microcentrifuge -
--PCR Thermal Cycler -
--Micropipettes & Tips -
--PCR Tubes 1/sample -
--Microcentrifuge Tubes 1/sample -
--Vortex -
--Heat Block or Water Bath -
--Agarose for ~0.8% - 1% gel -
--TAE Buffer -
--DNA Dye (recommended in gel, UV visualized dye) -
--Electrophoresis Aparatus -
--UV Lamp / UV Light Box -
--Scissors and Tweezers -
Cat # Product Name Description Size List Price
3000
EduPrimer™ DNA Isolation Kit

DNA Isolation components only (Solution A, B and Swabs)

30 rxns
$55
3001
EduPrimer™ DNA Profiling Kit - TPA-25

Full kit, as detailed in kit components, using TPA-25 primers

30 rxns
$150
3001R
EduPrimer™ PCR Reaction Kit - TPA-25

PCR reaction components only using TPA-25 primers.

30 rxns

$105

3001C
Positive Control for EduPrimer™ DNA Profiling Kit - TPA-25

Extra positive control DNA for TPA-25

10 rxns
$30
3002
EduPrimer™ DNA Profiling Kit - PV92

Full kit, as detailed in kit components, using PV92 primers

30 rxns
$150
3002R
EduPrimer™ PCR Reaction Kit - PV92

PCR reaction components only using PV92 primers.

30 rxns
$105

3002C

Positive Control for EduPrimer™ DNA Profiling Kit - PV92 Positive control DNA for PV92
10 rxns

$30

3010

DNA ladder Extra DNA ladder 100 µL

$42

User Manual click to download Download
Single Page Protocol click to download Download
Videos

We've put together short, 4 1/2 minute video showing kit components and the protocol:

Arizona State University also made a video describing their experience with the kit:

References

Detection of Alu by PCR: A Human DNA Fingerprinting Lab Protocol. DNA Learning Center. Cold Spring Harbor Laboratory. 1994.

Pray, Leslie A. Functions and Utility of Alu Jumping Genes. http://www.nature.com/scitable/topicpage/functions-and-utility-of-alu-jumping-genes-561. Nature Education (2008)

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